References Cited on this Website

By clicking on the titles you will be taken to a website where you may view the abstract or full version of the cited paper. Please note: full text of some papers may only be available on a pay-per-download basis unless you are accessing the page from an authorized site such as an educational institution with online access rights.

Zuk,P.A. et al. Multilineage cells from human adipose tissue: implications for cell- based therapies. Tissue Eng 7, 211-28 (2001)

This paper was the first to suggest that adipose tissue contains stem cells. In this work Dr Hedrick and his collaborators demonstrated that they could extract from human adipose tissue cells that are capable forming bone, cartilage, fat, and skeletal muscle. Later work by Dr Hedrick’s team showed that single cells were able to form all of these tissue types; that is, they showed that adipose tissue contains stem cells. Please click here to be taken to this follow-up work.

Kern,S., Eichler,H., Stoeve,J., Kluter,H. & Bieback,K. Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells 24, 1294-1301 (2006)

In this paper the authors compared stem cells isolated from human adipose tissue, bone marrow, and umbilical cord blood. Using a variation of a widely used assay for stem cell growth (CFU-F assay) they found that the frequency of stem cells in adipose tissue was approximately 250,000 times greater than that in umbilical cord blood and at least 7 times greater than bone marrow. Other studies have shown that the frequency of stem cells in adipose tissue is even higher than that reported by Kern et al in this study. Click here and here for links to these other studies.

Kitamura K, et al (2007) Stem cell augemented reconstruction: a new hope for reconstruction after breast conservation therapy. Breast Cancer Res Treat 2007; 106 (Supp1): Abstract 4071.

Please click here for an news article reporting the paper or click here for the full-length PDF including the abstract. The abstract can be found on page 238 of the PDF.

Serruys, P.W. et al. (2007). Adipose derived cells: new kids on the block. EuroIntervention, 2, Suppl B:B26-B32.

In this review article Dr Serruys and his colleagues discuss the use of adipose tissue-derived cells for the treatment of acute myocardial ischemia (heart attack).

Garcia-Olmo,D. et al. A phase I clinical trial of the treatment of Crohn's fistula by adipose mesenchymal stem cell transplantation. Dis Colon Rectum 48, 1416-1423 (2005).

In this paper Dr Garcia-Olmo and colleagues describe results of a Phase I human clinical trial using adipose tissue-derived stem cells in the treatment of fistulas associated with Crohn’s Disease. Crohn’s Disease is a form of inflammatory bowel disease that can result in the creation of fistulas (abnormal tunnels between two tissues) that are very hard to treat. In this paper Dr Garcia-Olmo has shown that treatment with adipose tissue-derived stem cells can successfully treat these fistulas.

Fraser,J.K., Wulur,I., Alfonso,Z. & Hedrick,M.H. Fat tissue: an underappreciated source of stem cells for biotechnology. Trends Biotechnol 24, 150-154 (2006).

In this paper the authors review the field of adipose tissue-derived stem cells. They present data generated with the widely-used CFU-F assay that shows that the frequency of stem cells in adipose tissue is approximately 1 stem cell for every 100 total cells. This finding is consistent with an independent study performed by others showing a stem cell frequency of approximately 1 stem cell for every 5 to 1 in every 8 total cells.

D'Ippolito,G., Schiller,P.C., Ricordi,C., Roos,B.A. & Howard,G.A. Age-related osteogenic potential of mesenchymal stromal stem cells from human vertebral bone marrow. J Bone Miner Res 14, 1115-22 (1999).

In this paper the authors used the CFU-AlkPhos assay to evaluate the number of bone stem cells in 41 donors aged from 3 to 70 years. Their results show a progressive decline in the number of bone stem cells with age.

Rossi,D.J. et al. Cell intrinsic alterations underlie hematopoietic stem cell aging. Proc Natl Acad Sci U S A 102, 9194-9199 (2005).

In this paper the authors purified blood stem cells and evaluated their function and gene expression. They demonstrate an age-associated decrease in function. In particular, the authors note that their data support the concept that stem cell aging contributes to age-dependent decline in immune function.

Nishida,S., Endo,N., Yamagiwa,H., Tanizawa,T. & Takahashi,H.E. Number of osteoprogenitor cells in human bone marrow markedly decreases after skeletal maturation. J Bone Miner Metab 17, 171-7 (1999).

In this paper the authors used the CFU-F and CFU-AlkPhos assays to evaluate bone stem cell content. Their data are consistent with the study by D’Ippolito et al published the same year in showing a decline in stem cell number with age.

Torella,D. et al. Cardiac stem cell and myocyte aging, heart failure, and insulin-like growth factor-1 overexpression. Circ. Res 94, 514-524 (2004).

In this study the authors modulated expression of key genes to look at the effects of aging on the properties of cardiac (heart) stem cells. They show that cardiac stem cell death and ability to generate new cardiac cells decreases with age and proposed that this phenomenon contributes to age-associated cardiomyopathy.

Oedayrajsingh-Varma,M.J. et al. Adipose tissue-derived mesenchymal stem cell yield and growth characteristics are affected by the tissue-harvesting procedure. Cytotherapy 8, 166-177 (2006).

In this paper the authors describe their studies on the different ways adipose tissue can be harvested and how they affect stem cell yield. As part of this study the authors cryopreserved the stem cells and evaluated function in thawed cells. Their data demonstrate that adipose tissue-derived stem cells can be frozen and thawed without impairing stem cell function.

Karlsson,J.O. & Toner,M. Long-term storage of tissues by cryopreservation: critical issues. Biomaterials 17, 243-56 (1996).

In this review article the authors discuss the science of cell and tissue cryopreservation and note that the temperature of liquid nitrogen is such that theoretical storage duration of up to 10,000 years is theoretically achievable.

Goh,B.C., Thirumala,S., Kilroy,G., Devireddy,R.V. & Gimble,J.M. Cryopreservation characteristics of adipose-derived stem cells: maintenance of differentiation potential and viability. J Tissue Eng Regen Med 1, 322-324 (2007).

In this study Goh et al show that adipose tissue-derived stem cells can be frozen and thawed without loss of viability or function.

Sharpless,N.E. & DePinho,R.A. How stem cells age and why this makes us grow old. Nat Rev Mol Cell Biol 8, 703-713 (2007).

This paper provides a detailed review of the molecular events and cellular changes associated with stem cell aging and discusses how such changes are manifested in human aging.

Meliga,E., Strem,B.M., Duckers,H.J. & Serruys,P.W. Adipose-derived cells. Cell Transplant 16, 963-970 (2007).

This paper summarizes some of the data that led to the development of a clinical trial that uses ADRC for heart disease.